Analysis Note

Positive ControlMCF7 cells

Application

Frozen Sections (see application reference)r>Immunoblotting (2 µg/ml)r>Immunofluorescence (see application reference)r>Immunoprecipitation (see application reference)r>Paraffin Sections (see application reference)

General description

Recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not cross-react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein.

Anti-BRCA1 (Ab-1), mouse monoclonal, clone MS110, recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. It is validated for WB, IF, IP, and frozen and paraffin sections.

Immunogen

recombinant human BRCA1

Epitope: within the N-terminal 304 amino acids of BRCA1

Human

Legal Information

Sold under license of U.S. Patents 5,753,441 and 6,162,897.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Scully, R., et al. 1996. Science272, 123.Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717.Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711.Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet.11, 113.Merajver, S.D., et al. 1995. Clin. Can. Res.1, 539.Merajver, S.D., et al. 1995. Nat. Genet.9, 439.Struewing, J.P., et al. 1995. Nat. Genet.11, 198.Thompson, M.E., et al. 1995. Nat. Genet.9, 444.Futreal, P.A., et al. 1994. Science266, 120.Miki, Y., et al. 1994. Science266, 66.Wilson, C.A., et al. 1999. Nat. Genet.21, 236.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)